2024 Pb7wgf2

Pb7wgf2

Author: rkmt

August undefined, 2024

Splet1.构建10kb大小，具有绿色荧光蛋白的质粒载体pb7wgf2.0； 2.用qiagen公司生产的qiafilter tm plasmidmidikit试剂盒制备质粒dna；设置质粒dna浓度为600ng/μl； 3.用sigma-aldrich … Splet19. dec. 2024 · A conserved residue in Stphot1, Asp at position 832 (D832), was mutated to Asn (N) generating Stphot1.KD, which results in a kinase-dead version of Stphot1. The mutant Stphot1.KD was recombined, using LR clonase (Invitrogen), into pB7WGF2 or pK7WGR2 vectors for in planta expression and in vitro kinase assays. Agrobacterium …

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Splet11. apr. 2024 · A binary vector pB7WGF2 and BastaRHindIISacICaMV Promoter SpeI eGFP GTW terminator were used for the construction of the vectors pRCH1::GFP-EBF2, … Splet03. apr. 2024 · The full AtMiro2 coding sequence was cloned using Gateway reactions (Invitrogen) into the binary destination vector PB7WGF2 creating an N-terminal GFP fusion. Using this as template, the ... military discount beach vacations

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Splet07. jul. 2024 · For transient expression of GFP-fusion proteins, the genomic sequences of GRP7, PTB3, RH11, a putative TNTase and a putative endonuclease were amplified from genomic DNA and cloned into pB7WGF2. DCP5 was amplified from genomic DNA, RH6, RH8 and RH12 coding sequences were amplified from cDNA and all cloned in pUB10-GFP. SpletThe primer sequences listed on the left are provided for your reference. Addgene does not distribute primers. For sequencing plasmids in our repository, we've chosen primers … Splet21. nov. 2024 · The GFP-OsGRXS17 construct was produced by an LR clonase reaction between the entry vector and pB7WGF2 51. As a control, a modified green fluorescent protein construct (Free GFP construct) was ... military discount big bear

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Pb7wgf2

Splet23. okt. 2024 · For SlACRE75, SlACRE180, NbACRE75 and NbACRE180, cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and transferred by a recombinant LR reaction of Gateway cloning (Clonase II enzyme mix Kit, Thermo Fisher) into pB7WGF2 (Karimi et al., 2002). Transient expression in Nicotiana … Splet01. jan. 2002 · To further verify our optimised protocol for PPT selection, we transformed BY-2 cells with another plasmid (pB7WGF2_GFP-SCO3) derived from the GATEWAY™ …

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Splet19. jun. 2024 · Agrobacterium tumefaciens containing overexpression vector pB7WGF2 was used to transform leaf discs of N. benthamiana. Positive lines were first screened on … Splet16. okt. 2024 · Agrobacterium tumefaciens carrying plasmids to express mRFP or GFP (empty pB7WGF2, or pB7WGR2 respectively) were infiltrated into the air spaces of one …

Splet01. jan. 2002 · To further verify our optimised protocol for PPT selection, we transformed BY-2 cells with another plasmid (pB7WGF2_GFP-SCO3) derived from the GATEWAY™ destination vector (Karimi et al. 2002 ... SpletpB7WGF2 plasmid vector植物表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心. 价格： ¥29865. 货号： pB7WGF2 plasmid vector植物表达载体质粒. 产地：北京. 点击 …

Splet23. jan. 2014 · Next, the AL6 endogenous promoter sequence (1311 bp upstream of the start codon) was PCR-amplified and used to replace the 35S promoter in pB7WGF2:GFP-AL6 by cloning into HindII and SpeI restriction sites, resulting in pAL6:GFP-AL6. pAL6:GFP-AL6 plasmid was introduced into Agrobacterium tumefaciens (GV3101) and the resulting … Splet03. mar. 2024 · For colocalization, pB7WGF2 plasmids containing EGFP-CRN C-terminal construct fusions and pGWB461 plasmids containing tagRFP-SlTCP14 fusions were transformed into Agrobacterium tumefaciens strain AGL1. For BiFC, CRN and TCP constructs were transformed into pCL112 and pCL113. Transformants were grown on …

Splet11. apr. 2024 · The gaseous hormone ethylene modulates many aspects of plant biology ranging from vegetative development, control of ripening, abscission, senescence, and reproduction to stress responses (1, 2).In most plant tissues, ethylene causes growth reduction due to inhibition of cell expansion, in both roots and shoots (3, 4), acting by …

Splet21. nov. 2024 · The GFP-OsGRXS17 construct was produced by an LR clonase reaction between the entry vector and pB7WGF2 51. As a control, a modified green fluorescent … new york pebtSpletLOG IN. Minimum purchase order is 150,00€. Free shipping on all products. Leave this field blank. Gateway vectors new york penal law 120.00Splet05. jul. 2024 · The pB7WGF2-WRKY33, pB7WGF2-APX1 and pB7WGF2-APX2 constructs were transformed into Agrobacterium tumefaciens strain GV3105 via electroporation. … military discount budget car rentalSplet20. okt. 2024 · An extract of N. benthamiana leaves expressing GFP alone (pB7WGF2.0) was prepared similarly and served as mock control for treatments. For monitoring hypersensitive responses ... new york penal codesSplet23. okt. 2024 · For SlACRE75, SlACRE180, NbACRE75 and NbACRE180, cDNAs from pUC57 entry vector were transformed by electroporation into Escherichia coli strain DH10B and … military discount broadway ticketsSplet19. dec. 2024 · The mutant Stphot1.KD was recombined, using LR clonase (Invitrogen), into pB7WGF2 or pK7WGR2 vectors for in planta expression and in vitro kinase assays. … military discount cable and internetSpletThermo Fisher pb7wgf2 vector. Pb7wgf2 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, … new york peking duck